Detection of co-eluted peptides using database search methods.

ABSTRACT:

Background

Current experimental techniques, especially those applying liquid chromatography mass spectrometry, have made high-throughput proteomic studies possible. The increase in throughput however also raises concerns on the accuracy of identification or quantification. Most experimental procedures select in a given MS scan only a few relatively most intense parent ions, each to be fragmented (MS2) separately, and most other minor co-eluted peptides that have similar chromatographic retention times are ignored and their information lost.

Results

We have computationally investigated the possibility of enhancing the information retrieval during a given LC/MS experiment by selecting the two or three most intense parent ions for simultaneous fragmentation. A set of spectra is

SUBMITTER: Alves G 

PROVIDER: S-EPMC2483259 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature