Identification of a set of genes showing regionally enriched expression in the mouse brain.

ABSTRACT:

Background

The Pleiades Promoter Project aims to improve gene therapy by designing human mini-promoters (< 4 kb) that drive gene expression in specific brain regions or cell-types of therapeutic interest. Our goal was to first identify genes displaying regionally enriched expression in the mouse brain so that promoters designed from orthologous human genes can then be tested to drive reporter expression in a similar pattern in the mouse brain.

Results

We have utilized LongSAGE to identify regionally enriched transcripts in the adult mouse brain. As supplemental strategies, we also performed a meta-analysis of published literature and inspected the Allen Brain Atlas in situ hybridization data. From a set of approximately 30,000 mouse genes, 237 were identified as showing spec

SUBMITTER: D'Souza CA 

PROVIDER: S-EPMC2483290 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature