Heat stress enhances the accumulation of polyadenylated mitochondrial transcripts in Arabidopsis thaliana.

ABSTRACT:

Background

Polyadenylation of RNA has a decisive influence on RNA stability. Depending on the organisms or subcellular compartment, it either enhances transcript stability or targets RNAs for degradation. In plant mitochondria, polyadenylation promotes RNA degradation, and polyadenylated mitochondrial transcripts are therefore widely considered to be rare and unstable. We followed up a surprising observation that a large number of mitochondrial transcripts are detectable in microarray experiments that used poly(A)-specific RNA probes, and that these transcript levels are significantly enhanced after heat treatment.

Methodology/principal findings

As the Columbia genome contains a complete set of mitochondrial genes, we had to identify polymorphisms to differentiate between nu

SUBMITTER: Adamo A 

PROVIDER: S-EPMC2483354 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature