High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome.

ABSTRACT:

Background

Benefits from high-throughput sequencing using 454 pyrosequencing technology may be most apparent for species with high societal or economic value but few genomic resources. Rapid means of gene sequence and SNP discovery using this novel sequencing technology provide a set of baseline tools for genome-level research. However, it is questionable how effective the sequencing of large numbers of short reads for species with essentially no prior gene sequence information will support contig assemblies and sequence annotation.

Results

With the purpose of generating the first broad survey of gene sequences in Eucalyptus grandis, the most widely planted hardwood tree species, we used 454 technology to sequence and assemble 148 Mbp of expressed sequences (EST). EST sequen

SUBMITTER: Novaes E 

PROVIDER: S-EPMC2483731 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature