Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to Tat.

ABSTRACT:

Background

During HIV-1 infection, the Tat protein plays a key role by transactivating the transcription of the HIV-1 proviral DNA. In addition, Tat induces apoptosis of non-infected T lymphocytes, leading to a massive loss of immune competence. This apoptosis is notably mediated by the interaction of Tat with microtubules, which are dynamic components essential for cell structure and division. Tat binds two Zn2+ ions through its conserved cysteine-rich region in vitro, but the role of zinc in the structure and properties of Tat is still controversial.

Results

To investigate the role of zinc, we first characterized Tat apo- and holo-forms by fluorescence correlation spectroscopy and time-resolved fluorescence spectroscopy. Both of the Tat forms are monomeric and poorly folde

SUBMITTER: Egele C 

PROVIDER: S-EPMC2483996 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature