Proteomics reveals novel Drosophila seminal fluid proteins transferred at mating.

ABSTRACT: Across diverse taxa, seminal fluid proteins (Sfps) transferred at mating affect the reproductive success of both sexes. Such reproductive proteins often evolve under positive selection between species; because of this rapid divergence, Sfps are hypothesized to play a role in speciation by contributing to reproductive isolation between populations. In Drosophila, individual Sfps have been characterized and are known to alter male sperm competitive ability and female post-mating behavior, but a proteomic-scale view of the transferred Sfps has been missing. Here we describe a novel proteomic method that uses whole-organism isotopic labeling to detect transferred Sfps in mated female D. melanogaster. We identified 63 proteins, which were previously unknown to function in reproduction, and conf

SUBMITTER: Findlay GD 

PROVIDER: S-EPMC2486302 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature