A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in Medicago truncatula.

ABSTRACT:

Background

Medicago truncatula is a model legume species that is currently the focus of an international genome sequencing effort. Although several different oligonucleotide and cDNA arrays have been produced for genome-wide transcript analysis of this species, intrinsic limitations in the sensitivity of hybridization-based technologies mean that transcripts of genes expressed at low-levels cannot be measured accurately with these tools. Amongst such genes are many encoding transcription factors (TFs), which are arguably the most important class of regulatory proteins. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is the most sensitive method currently available for transcript quantification, and one that can be scaled up to analyze transcripts of thousands

SUBMITTER: Kakar K 

PROVIDER: S-EPMC2490690 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature