Silencer elements controlling the B29 (Igbeta) promoter are neither promoter- nor cell-type-specific.

ABSTRACT: The murine B29 (Igbeta) promoter is B cell specific and contains essential SP1, ETS, OCT, and Ikaros motifs. Flanking 5' DNA sequences inhibit B29 promoter activity, suggesting this region contains silencer elements. Two adjacent 5' DNA segments repress transcription by the murine B29 promoter in a position- and orientation-independent manner, analogous to known silencers. Both these 5' segments also inhibit transcription by several heterologous promoters in B cells, including mb-1, c-fos, and human B29. These 5' segments also inhibit transcription by the c-fos promoter in T cells suggesting they are not B cell-specific elements. DNase I footprint analyses show an approximately 70-bp protected region overlapping the boundary between the two negative regulatory DNA segments and correspondin

SUBMITTER: Malone CS 

PROVIDER: S-EPMC24921 | biostudies-literature | 1997 Nov

REPOSITORIES: biostudies-literature