Differential expression of the Smb bacteriocin in Streptococcus mutans isolates.

ABSTRACT: The two-component lantibiotic Smb is produced by Streptococcus mutans GS5. In the present study, we identified seven strains of S. mutans containing the smb gene cluster. These strains could be classified into high- and low-level Smb producers relative to the levels of Smb production by indicator strains in vitro. This classification was dependent upon the transcription levels of the structural smbA and smbB genes. Sequence analysis upstream of smbA in the high- and low-level Smb-producing strains revealed differences at nucleotide position -46 relative to the smbA start codon. Interestingly, the transcription start site was present upstream of the point mutation, indicating that both groups of strains have the same promoter constructs and that the differential expression of smbA and smbB

SUBMITTER: Yonezawa H 

PROVIDER: S-EPMC2493135 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature