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Analysis of vaginal lactobacilli from healthy and infected Brazilian women.


ABSTRACT: Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.

SUBMITTER: Martinez RC 

PROVIDER: S-EPMC2493183 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Analysis of vaginal lactobacilli from healthy and infected Brazilian women.

Martinez Rafael C R RC   Franceschini Sílvio A SA   Patta Maristela C MC   Quintana Silvana M SM   Nunes Alvaro C AC   Moreira João L S JL   Anukam Kingsley C KC   Reid Gregor G   De Martinis Elaine C P EC  

Applied and environmental microbiology 20080523 14


Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC. ...[more]

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