In vivo analysis of Aspergillus fumigatus developmental gene expression determined by real-time reverse transcription-PCR.

ABSTRACT: Very little is known about the developmental stages of Aspergillus fumigatus during invasive aspergillosis. We performed real-time reverse transcription-PCR analysis on lung samples from mice with invasive pulmonary aspergillosis to determine the expression of A. fumigatus genes that are expressed at specific stages of development. In established infection, A. fumigatus exhibited mRNA expression of genes specific to developmentally competent hyphae, such as stuA. In contrast, mRNA of genes expressed by conidia and precompetent hyphae was not detected. Many genes required for mycotoxin synthesis, including aspHS, gliP, mitF, and metAP, are known to be expressed by developmentally competent hyphae in vitro. Interestingly, each of these genes was expressed at significantly higher levels durin

SUBMITTER: Gravelat FN 

PROVIDER: S-EPMC2493243 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature