A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning.

ABSTRACT: Using the massively parallel technique of sequencing by oligonucleotide ligation and detection (SOLiD; Applied Biosystems), we have assessed the in vivo positions of more than 44 million putative nucleosome cores in the multicellular genetic model organism Caenorhabditis elegans. These analyses provide a global view of the chromatin architecture of a multicellular animal at extremely high density and resolution. While we observe some degree of reproducible positioning throughout the genome in our mixed stage population of animals, we note that the major chromatin feature in the worm is a diversity of allowed nucleosome positions at the vast majority of individual loci. While absolute positioning of nucleosomes can vary substantially, relative positioning of nucleosomes (in a repeated array

SUBMITTER: Valouev A 

PROVIDER: S-EPMC2493394 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature