Synthetic antibody libraries focused towards peptide ligands.

ABSTRACT: Synthetic antibody libraries have proven immensely useful for the de novo isolation of antibodies without the need for animal immunization. Recently, focused libraries designed to recognize particular classes of ligands, such as haptens or proteins, have been employed to facilitate the selection of high-affinity antibodies. Focused libraries are built using V regions encoding combinations of canonical structures that resemble the structural features of antibodies that bind the desired class of ligands and sequence diversity is introduced at residues typically involved in recognition. Here we describe the generation and experimental validation of two different single-chain antibody variable fragment libraries that efficiently generate binders to peptides, a class of molecules that has prove

SUBMITTER: Cobaugh CW 

PROVIDER: S-EPMC2494707 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature