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Cloning, expression and purification of cytochrome c(6) from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure.

ABSTRACT: The primary sequence of cytochrome c(6) from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 A resolution. The crystal belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 84.58, c = 232.91 A and six molecules per asymmetric unit. The genome code, amino-acid sequence and crystal structure of H. fusiformis cytochrome c(6) were most similar to those of red algal cytochrome c(6). These results support the hypothesis that brown algae acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host.

SUBMITTER: Akazaki H

PROVIDER: S-EPMC2494970 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Cloning, expression and purification of cytochrome c(6) from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure.

Akazaki Hideharu H Kawai Fumihiro F Chida Hirotaka H Matsumoto Yuichirou Y Hirayama Mao M Hoshikawa Ken K Unzai Satoru S Hakamata Wataru W Nishio Toshiyuki T Park Sam Yong SY Oku Tadatake T

Acta crystallographica. Section F, Structural biology and crystallization communications 20080705 Pt 8

The primary sequence of cytochrome c(6) from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 A resolution. The crystal belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 84.58, c = 232.91 A and six molecules per asymmetric unit. The genome code, amino-acid sequence and crystal structure of H. fusiformis cytochrome c(6) were most similar to those of red algal cytochrome c(6). These results sup ...[more]

PMID: 18678931

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