Characterization of eight novel proteins with male germ cell-specific expression in mouse.

ABSTRACT:

Background

Spermatogenesis and fertilization are highly unique processes. Discovery and characterization of germ cell-specific genes are important for the understanding of these reproductive processes. We investigated eight proteins encoded by novel spermatogenic cell-specific genes previously identified from the mouse round spermatid UniGene library.

Methods

Polyclonal antibodies were generated against the novel proteins and western blot analysis was performed with various protein samples. Germ cell specificity was investigated using testes from germ cell-less mutant mice. Developmental expression pattern was examined in testicular germ cells, testicular sperm and mature sperm. Subcellular localization was assessed by cell surface biotin labeling and trypsinization. Protein

SUBMITTER: Baek N 

PROVIDER: S-EPMC2500023 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature