Short tandem repeat sequences in the Mycoplasma genitalium genome and their use in a multilocus genotyping system.

ABSTRACT:

Background

Several methods have been reported for strain typing of Mycoplasma genitalium. The value of these methods has never been comparatively assessed. The aims of this study were: 1) to identify new potential genetic markers based on an analysis of short tandem repeat (STR) sequences in the published M. genitalium genome sequence; 2) to apply previously and newly identified markers to a panel of clinical strains in order to determine the optimal combination for an efficient multi-locus genotyping system; 3) to further confirm sexual transmission of M. genitalium using the newly developed system.

Results

We performed a comprehensive analysis of STRs in the genome of the M. genitalium type strain G37 and identified 18 loci containing STRs. In addition to one previously st

SUBMITTER: Ma L 

PROVIDER: S-EPMC2515158 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature