Optical mapping discerns genome wide DNA methylation profiles.

ABSTRACT:

Background

Methylation of CpG dinucleotides is a fundamental mechanism of epigenetic regulation in eukaryotic genomes. Development of methods for rapid genome wide methylation profiling will greatly facilitate both hypothesis and discovery driven research in the field of epigenetics. In this regard, a single molecule approach to methylation profiling offers several unique advantages that include elimination of chemical DNA modification steps and PCR amplification.

Results

A single molecule approach is presented for the discernment of methylation profiles, based on optical mapping. We report results from a series of pilot studies demonstrating the capabilities of optical mapping as a platform for methylation profiling of whole genomes. Optical mapping was used to discern the

SUBMITTER: Ananiev GE 

PROVIDER: S-EPMC2516518 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature