Fluorescent pyrimidine ribonucleotide: synthesis, enzymatic incorporation, and utilization.

ABSTRACT: Fluorescent nucleobase analogues that respond to changes in their microenvironment are valuable for studying RNA structure, dynamics, and recognition. The most commonly used fluorescent ribonucleoside is 2-aminopurine, a highly responsive purine analogue. Responsive isosteric fluorescent pyrimidine analogues are, however, rare. Appending five-membered aromatic heterocycles at the 5-position on a pyrimidine core has recently been found to provide a family of responsive fluorescent nucleoside analogues with emission in the visible range. To explore the potential utility of this chromophore for studying RNA-ligand interactions, an efficient incorporation method is necessary. Here we describe the synthesis of the furan-containing ribonucleoside and its triphosphate, as well as their basic phot

SUBMITTER: Srivatsan SG 

PROVIDER: S-EPMC2517582 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature