Cell membrane proteomic analysis identifies proteins differentially expressed in osteotropic human breast cancer cells.

ABSTRACT: Metastatic breast cancer cells are characterized by their high propensity to colonize the skeleton and form bone metastases, causing major morbidity and mortality. Identifying key proteins involved in the osteotropic phenotype would represent a major step toward the development of both new prognostic markers and new effective therapies. Cell surface proteins differentially expressed in cancer cells are preferred potential targets for antibody-based targeted therapies. In this study, using cell surface biotinylation and a mass spectrometric approach, we have compared the profile of accessible cell surface proteins between the human breast cancer cell line MDA-MB-231 and its highly osteotropic B02 subclone. This strategy allowed the identification of several proteins either up- or downregula

SUBMITTER: Kischel P 

PROVIDER: S-EPMC2517647 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature