Pyrosequence analysis of expressed sequence tags for Manduca sexta hemolymph proteins involved in immune responses.

ABSTRACT: The tobacco hornworm Manduca sexta is widely used as a model organism to investigate the biochemical basis of insect physiological processes but little transcriptome information is available. To get a broad view of the larval hemolymph proteins, particularly those related to immunity, we synthesized and sequenced cDNA fragments from a mixture of eight total RNA samples: fat body and hemocytes from larvae injected with killed bacteria, fat body, hemocytes, integument and trachea from naïve larvae, and fat body and hemocytes from wandering larvae. Using massively parallel pyrosequencing, we obtained 95,458 M. sexta expressed sequence tags (ESTs) at an average size of 185bp per read. A majority of the sequences (69,429 reads) could be assembled into 7231 contigs with an average size of 300bp,

SUBMITTER: Zou Z 

PROVIDER: S-EPMC2517850 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature