A translation initiation element specific to mRNAs with very short 5'UTR that also regulates transcription.

ABSTRACT: Transcription is controlled by cis regulatory elements, which if localized downstream to the transcriptional start site (TSS), in the 5'UTR, could influence translation as well. However presently there is little evidence for such composite regulatory elements. We have identified by computational analysis an abundant element located downstream to the TSS up to position +30, which controls both transcription and translation. This element has an invariable ATG sequence, which serves as the translation initiation codon in 64% of the genes bearing it. In these genes the initiating AUG is preceded by an extremely short 5'UTR. We show that translation in vitro and in vivo is initiated exclusively from the AUG of this motif, and that the AUG flanking sequences create a strong translation initiatio

SUBMITTER: Elfakess R 

PROVIDER: S-EPMC2518114 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature