Interaction of the growth and tumour suppressor NORE1A with microtubules is not required for its growth-suppressive function.

ABSTRACT:

Background

The NORE1 protein was identified in a yeast two-hybrid screen as a Ras effector that binds Ras protein in a GTP-dependent manner. NORE1A is a growth and tumour suppressor that is inactivated in a variety of cancers. In transformed human cells, both full-length NORE1A protein and its effector domain alone (amino acids 191-363) are localized to microtubules and centrosomes. However, the mechanism by which NORE1A associates with these cytoskeletal elements is not known; furthermore, whether centrosomally-associated or microtubule-associated NORE1A suppresses tumour cell growth has not been yet established.

Findings

We have shown that purified NORE1A fails to bind to microtubules in vitro suggesting that other protein(s) mediate NORE1A-microtubule association. Using m

SUBMITTER: Moshnikova A 

PROVIDER: S-EPMC2518271 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature