Detection of large numbers of novel sequences in the metatranscriptomes of complex marine microbial communities.

ABSTRACT:

Background

Sequencing the expressed genetic information of an ecosystem (metatranscriptome) can provide information about the response of organisms to varying environmental conditions. Until recently, metatranscriptomics has been limited to microarray technology and random cloning methodologies. The application of high-throughput sequencing technology is now enabling access to both known and previously unknown transcripts in natural communities.

Methodology/principal findings

We present a study of a complex marine metatranscriptome obtained from random whole-community mRNA using the GS-FLX Pyrosequencing technology. Eight samples, four DNA and four mRNA, were processed from two time points in a controlled coastal ocean mesocosm study (Bergen, Norway) involving an induced phy

SUBMITTER: Gilbert JA 

PROVIDER: S-EPMC2518522 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature