Optically switchable chelates: optical control and sensing of metal ions.

ABSTRACT: This study introduces new concepts in the design, synthesis, and in vitro and in vivo characterization, manipulation, and imaging of organic chelates whose association with metal ions is rapidly and reversibly controlled by using light. Di- and tricarboxylic group bearing photochromes, nitrobenzospiropyran (nitroBIPS), undergo rapid and reversible, optically driven transitions between their spiro (SP) and fluorescent merocyanine (MC) states. The MC state of nitroBIPS-8-DA binds tightly to various metal ions resulting in specific shifts in absorption and fluorescence, and the dissociation constant for its Gadolinium complex in water is measured at approximately 5 microM. The metal-bound MC state is converted to the weaker-binding SP state with use of 543 nm light, while the SP to MC transit

SUBMITTER: Sakata T 

PROVIDER: S-EPMC2518625 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature