Identification of neural crest and glial enhancers at the mouse Sox10 locus through transgenesis in zebrafish.

ABSTRACT: Sox10 is a dynamically regulated transcription factor gene that is essential for the development of neural crest-derived and oligodendroglial populations. Developmental genes often require multiple regulatory sequences that integrate discrete and overlapping functions to coordinate their expression. To identify Sox10 cis-regulatory elements, we integrated multiple model systems, including cell-based screens and transposon-mediated transgensis in zebrafish, to scrutinize mammalian conserved, noncoding genomic segments at the mouse Sox10 locus. We demonstrate that eight of 11 Sox10 genomic elements direct reporter gene expression in transgenic zebrafish similar to patterns observed in transgenic mice, despite an absence of observable sequence conservation between mice and zebrafish. Multiple

SUBMITTER: Antonellis A 

PROVIDER: S-EPMC2518861 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature