Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis.

ABSTRACT:

Background

All aerobically grown living cells are exposed to oxidative damage by reactive oxygen species (ROS). A major damage by ROS to proteins is caused by covalent modifications of methionine residues giving methionine sulfoxide (Met-SO). Methionine sulfoxide reductases are enzymes able to regenerate methionine and restore protein function after oxidative damage.

Results

We characterized the methionine sulfoxide reductase genes msrA and msrB in Bacillus subtilis, forming an operon transcribed from a single sigma A-dependent promoter. The msrAB operon was specifically induced by oxidative stress caused by paraquat (PQ) but not by H2O2. Spx, a global oxidative stress regulator in B. subtilis, is primarily responsible for this PQ-specific induction of msrAB expression. In s

SUBMITTER: You C 

PROVIDER: S-EPMC2518928 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature