Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels.

ABSTRACT: Combining single-vessel perfusion technique with confocal microscopy, this study presents a new approach that allows three-dimensional visualization and quantification of endothelial gaps under experimental conditions identical to those used to measure permeability coefficients, endothelial calcium concentration, and nitric oxide production in individually perfused intact microvessels. This approach provides an efficient means for defining the transport pathways and cellular mechanisms of increased microvascular permeability during inflammation. Platelet-activating factor (PAF) was used to increase the permeability of individually perfused rat mesenteric venules. Fluorescent microspheres (FMs, 100 nm) were used as leakage markers, and confocal images were acquired at successive focal plane

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC2519217 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature