Nonlabeled quartz crystal microbalance biosensor for bacterial detection using carbohydrate and lectin recognitions.

ABSTRACT: High percentages of harmful microbes or their secreting toxins bind to specific carbohydrate sequences on human cells at the recognition and attachment sites. A number of studies also show that lectins react with specific structures of bacteria and fungi. In this report, we take advantage of the fact that a high percentage of microorganisms have both carbohydrate and lectin binding pockets at their surface. We demonstrate here for the first time that a carbohydrate nonlabeled mass sensor in combination with lectin-bacterial O-antigen recognition can be used for detection of high molecular weight bacterial targets with remarkably high sensitivity and enhanced specificity. A functional mannose self-assembled monolayer in combination with lectin concanavalin A (Con A) was used as molecular re

SUBMITTER: Shen Z 

PROVIDER: S-EPMC2519234 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature