High-throughput identification and validation of in situ-expressed genes of Lactococcus lactis.

ABSTRACT: Understanding the functional response of bacteria to their natural environment is one of the current challenges in microbiology. Over the past decades several techniques have been developed to study gene expression in complex natural habitats. Most of these methods, however, are laborious, and validation of results under in situ conditions is cumbersome. Here we report the improvement of the recombinase-based in vivo expression technology (R-IVET) by the implementation of two additional reporter genes. The first one is an alpha-galactosidase gene (melA), which facilitates the rapid identification of in vivo-induced genes. Second, the bacterial luciferase genes (luxAB) are transcriptionally coupled to the resolvase gene, which allows rapid validation and characterization of in vivo-induced

SUBMITTER: Bachmann H 

PROVIDER: S-EPMC2519351 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature