Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide.

ABSTRACT: In order to gain insight into the effects of human breast milk on the development of the intestinal bifidobacteria and associated health effects, the transcriptome of Bifidobacterium longum LMG 13197 grown in breast milk and formula milk containing galactooligosaccharides (GOS) and long-chain fructooligosaccharides was compared to that obtained in a semisynthetic medium with glucose. Total RNA was isolated from exponentially growing cells and hybridized to a clone library-based microarray. Inserts of clones with significant hybridization signals were sequenced and identified. The B. longum transcriptomes obtained during growth on human and formula milk were more similar to each other than to that obtained from growth in semisynthetic medium with glucose. Remarkably, there were only a few g

SUBMITTER: Gonzalez R 

PROVIDER: S-EPMC2519361 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature