Expression of green fluorescent protein fused to magnetosome proteins in microaerophilic magnetotactic bacteria.

ABSTRACT: The magnetosomes of magnetotactic bacteria are prokaryotic organelles consisting of a magnetite crystal bounded by a phospholipid bilayer that contains a distinct set of proteins with various functions. Because of their unique magnetic and crystalline properties, magnetosome particles are potentially useful as magnetic nanoparticles in a number of applications, which in many cases requires the coupling of functional moieties to the magnetosome membrane. In this work, we studied the use of green fluorescent protein (GFP) as a reporter for the magnetosomal localization and expression of fusion proteins in the microaerophilic Magnetospirillum gryphiswaldense by flow cytometry, fluorescence microscopy, and biochemical analysis. Although optimum conditions for high fluorescence and magnetite sy

SUBMITTER: Lang C 

PROVIDER: S-EPMC2519370 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature