Identification of isomeric N-glycan structures by mass spectrometry with 157 nm laser-induced photofragmentation.

ABSTRACT: Characterization of structural isomers has become increasingly important and extremely challenging in glycobiology. This communication demonstrates the capability of ion-trap mass spectrometry in conjunction with 157 nm photofragmentation to identify different structural isomers of permethylated N-glycans derived from ovalbumin without chromatographic separation. The results are compared with collision-induced dissociation (CID) experiments. Photodissociation generates extensive cross-ring fragment ions as well as diagnostic glycosidic product ions that are not usually observed in CID MS/MS experiments. The detection of these product ions aids in characterizing indigenous glycan isomers. The ion trap facilitates MS(n) experiments on the diagnostic glycosidic fragments and cross-ring produc

SUBMITTER: Devakumar A 

PROVIDER: S-EPMC2523269 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature