Anillin-mediated targeting of peanut to pseudocleavage furrows is regulated by the GTPase Ran.

ABSTRACT: During early development in Drosophila, pseudocleavage furrows in the syncytial embryo prevent contact between neighboring spindles, thereby ensuring proper chromosome segregation. Here we demonstrate that the GTPase Ran regulates pseudocleavage furrow organization. Ran can exert control on pseudocleavage furrows independently of its role in regulating the microtubule cytoskeleton. Disruption of the Ran pathway prevented pseudocleavage furrow formation and restricted the depth and duration of furrow ingression of those pseudocleavage furrows that did form. We found that Ran was required for the localization of the septin Peanut to the pseudocleavage furrow, but not anillin or actin. Biochemical assays revealed that the direct binding of the nuclear transport receptors importin alpha and be

SUBMITTER: Silverman-Gavrila RV 

PROVIDER: S-EPMC2526685 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature