Genome-wide analysis of alternative promoters of human genes using a custom promoter tiling array.

ABSTRACT:

Background

Independent lines of evidence suggested that a large fraction of human genes possess multiple promoters driving gene expression from distinct transcription start sites. Understanding which promoter is employed in which cellular context is required to unravel gene regulatory networks within the cell.

Results

We have developed a custom microarray platform that tiles roughly 35,000 alternative putative promoters from nearly 7,000 genes in the human genome. To demonstrate the utility of this array platform, we have analyzed the patterns of promoter usage in 17beta-estradiol (E2)-treated and untreated MCF7 cells and show widespread usage of alternative promoters. Most intriguingly, we show that the downstream promoter in E2-sensitive multiple promoter genes tends to be

SUBMITTER: Singer GA 

PROVIDER: S-EPMC2527337 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature