Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis.

ABSTRACT:

Background

Anaplasma phagocytophilum (Ap) is an obligate intracellular bacterium and the agent of human granulocytic anaplasmosis, an emerging tick-borne disease. Ap alternately infects ticks and mammals and a variety of cell types within each. Understanding the biology behind such versatile cellular parasitism may be derived through the use of tiling microarrays to establish high resolution, genome-wide transcription profiles of the organism as it infects cell lines representative of its life cycle (tick; ISE6) and pathogenesis (human; HL-60 and HMEC-1).

Results

Detailed, host cell specific transcriptional behavior was revealed. There was extensive differential Ap gene transcription between the tick (ISE6) and the human (HL-60 and HMEC-1) cell lines, with far fewer differen

SUBMITTER: Nelson CM 

PROVIDER: S-EPMC2527338 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature