An optimized, chemically regulated gene expression system for Chlamydomonas.

ABSTRACT:

Background

Chlamydomonas reinhardtii is a model system for algal and cell biology and is used for biotechnological applications, such as molecular farming or biological hydrogen production. The Chlamydomonas metal-responsive CYC6 promoter is repressed by copper and induced by nickel ions. However, induction by nickel is weak in some strains, poorly reversible by chelating agents like EDTA, and causes, at high concentrations, toxicity side effects on Chlamydomonas growth. Removal of these bottlenecks will encourage the wide use of this promoter as a chemically regulated gene expression system.

Methodology

Using a codon-optimized Renilla luciferase as a reporter gene, we explored several strategies to improve the strength and reversibility of CYC6 promoter induction. Use of th

SUBMITTER: Ferrante P 

PROVIDER: S-EPMC2527658 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature