RCADiA: simple automation platform for comparative multidimensional protein identification technology.

ABSTRACT: Multidimensional liquid chromatography in combination with tandem mass spectrometry has been used to analyze a variety of biological structures including protein complexes. Incorporating this approach with autosampling devices presents a number of problems including decreased sensitivity due to exposure to extra surfaces, carryover from run to run, and increased dead volume. We developed a device, termed Radial Column Array for Distribution and Automation (RCADiA), to automate multiple MuDPIT experiments while eliminating many of these problems and maintaining a high resolution and sensitive analysis. The design, which places each sample downstream of any common fluid path, presents a low risk of carryover between successive analyses. Beyond the convenience of automation, the RCADiA platfo

SUBMITTER: Bailey AO 

PROVIDER: S-EPMC2528021 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature