Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina.

ABSTRACT:

Purpose

Intracellular free calcium ions (Ca(2+)) are an important element in retinal ganglion cell response. Two major EF-hand (E-helix-loop-F-helix-hand) calcium binding proteins in the retina, calretinin and calbindin-28 kDa, are important buffers of intracellular free Ca(2+) in neurons, and may also serve as Ca(2+)-dependent regulators of enzymes and ion channels.

Methods

This study used immunohistochemistry to investigate the subcellular expression patterns of calretinin and calbindin-28 kDa, in the soma, dendrites, and the axonal compartment of rat retinal ganglion cells.

Results

Antibodies for calretinin and calbindin-28 kDa labeled different cell populations in the retinal ganglion cell layer. In this layer, calretinin labeled a larger number of cells compared

SUBMITTER: Mojumder DK 

PROVIDER: S-EPMC2528027 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature