Functional studies on transfected cell microarray analysed by linear regression modelling.

ABSTRACT: Transfected cell microarray is a promising method for accelerating the functional exploration of the genome, giving information about protein function in the living cell. The microarrays consist of clusters of cells (spots) overexpressing or silencing a particular gene product. The subsequent analysis of the phenotypic consequences of such perturbations can then be detected using cell-based assays. The focus in the present study was to establish an experimental design and a robust analysis approach for fluorescence intensity data, and to address the use of replicates for studying regulation of gene expression with varying complexity and effect size. Our analysis pipeline includes measurement of fluorescence intensities, normalization strategies using negative control spots and internal con

SUBMITTER: Fjeldbo CS 

PROVIDER: S-EPMC2528170 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature