The splice variants of UBF differentially regulate RNA polymerase I transcription elongation in response to ERK phosphorylation.

ABSTRACT: The mammalian architectural HMGB-Box transcription factor UBF is ubiquitously expressed in two variant forms as the result of a differential splicing event, that in the UBF2 deletes 37 amino acid from the second of six HMGB-boxes. Several attempts to define a function for this shorter UBF2 protein have been less than satisfactory. However, since all mammals appear to display similar levels of the longer and shorter UBF variants, it is unlikely that UBF2 is simply nonfunctional. Previously we showed that phosphorylation of UBF by the MAP-kinase ERK regulates chromatin folding and transcription elongation, explaining the rapid response of the ribosomal RNA genes to growth factors. Here we have investigated the roles the UBF variants play in the response of these genes to ERK activity. We dem

SUBMITTER: Stefanovsky VY 

PROVIDER: S-EPMC2528179 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature