60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes.

ABSTRACT: During the highly conserved process of eukaryotic ribosome formation, RNA follows a maturation path with well-defined, successive intermediates that dynamically associate with many pre-ribosomal proteins. A comprehensive description of the assembly process is still lacking. To obtain data on the timing and order of association of the different pre-ribosomal factors, a strategy consists in the use of pre-ribsomal particles isolated from mutants that block ribosome formation at different steps. Immunoblots, inherently limited to only a few factors, have been applied to evaluate the accumulation or decrease of pre-ribosomal intermediates under mutant conditions. For a global protein-level description of different 60S ribosomal subunit maturation intermediates in yeast, we have adapted a metho

SUBMITTER: Lebreton A 

PROVIDER: S-EPMC2528192 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature