Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity.

ABSTRACT: [reaction: see text] Lysophospholipase D (lysoPLD), also known as autotaxin (ATX), is an important source of the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-3) show significant increases in fluorescence when treated with recombinant ATX and have potential applications in screening for this emerging drug target.

SUBMITTER: Ferguson CG 

PROVIDER: S-EPMC2528860 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature