Domain orientation in the inactive response regulator Mycobacterium tuberculosis MtrA provides a barrier to activation.

ABSTRACT: The structure of MtrA, an essential gene product for the human pathogen Mycobacterium tuberculosis, has been solved to a resolution of 2.1 A. MtrA is a member of the OmpR/PhoB family of response regulators and represents the fourth family member for which a structure of the protein in its inactive state has been determined. As is true for all OmpR/PhoB family members, MtrA possesses an N-terminal regulatory domain and a C-terminal winged helix-turn-helix DNA-binding domain, with phosphorylation of the regulatory domain modulating the activity of the protein. In the inactive form of MtrA, these two domains form an extensive interface that is composed of the alpha4-beta5-alpha5 face of the regulatory domain and the C-terminal end of the positioning helix, the trans-activation loop, and the r

SUBMITTER: Friedland N 

PROVIDER: S-EPMC2528954 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature