Integrating technologies for comparing 3D gene expression domains in the developing chick limb.

ABSTRACT: Chick embryos are good models for vertebrate development due to their accessibility and manipulability. Recent large increases in available genomic data from both whole genome sequencing and EST projects provide opportunities for identifying many new developmentally important chicken genes. Traditional methods of documenting when and where specific genes are expressed in embryos using whole amount and section in-situ hybridisation do not readily allow appreciation of 3-dimensional (3D) patterns of expression, but this can be accomplished by the recently developed microscopy technique, Optical Projection Tomography (OPT). Here we show that OPT data on the developing chick wing from different labs can be reliably integrated into a common database, that OPT is efficient in capturing 3D gene e

SUBMITTER: Fisher ME 

PROVIDER: S-EPMC2529376 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature