FAST-NMR: functional annotation screening technology using NMR spectroscopy.

ABSTRACT: An abundance of protein structures emerging from structural genomics and the Protein Structure Initiative (PSI) are not amenable to ready functional assignment because of a lack of sequence and structural homology to proteins of known function. We describe a high-throughput NMR methodology (FAST-NMR) to annotate the biological function of novel proteins through the structural and sequence analysis of protein-ligand interactions. This is based on basic tenets of biochemistry where proteins with similar functions will have similar active sites and exhibit similar ligand binding interactions, despite global differences in sequence and structure. Protein-ligand interactions are determined through a tiered NMR screen using a library composed of compounds with known biological activity. A rapid

SUBMITTER: Mercier KA 

PROVIDER: S-EPMC2529462 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature