Mutational analysis of highly conserved aspartate residues essential to the catalytic core of the piggyBac transposase.

ABSTRACT:

Background

The piggyBac mobile element is quickly gaining popularity as a tool for the transgenesis of many eukaryotic organisms. By studying the transposase which catalyzes the movement of piggyBac, we may be able to modify this vector system to make it a more effective transgenesis tool. In a previous publication, Sarkar A, Sim C, Hong YS, Hogan JR, Fraser MJ, Robertson HM, and Collins FH have proposed the presence of the widespread 'DDE/DDD' motif for piggyBac at amino acid positions D268, D346, and D447.

Results

This study utilizes directed mutagenesis and plasmid-based mobility assays to assess the importance of these residues as the catalytic core of the piggyBac transposase. We have functionally analyzed individual point-mutations with respect to charge and physical s

SUBMITTER: Keith JH 

PROVIDER: S-EPMC2533014 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature