An approach to analyse the specific impact of rapamycin on mRNA-ribosome association.

ABSTRACT:

Background

Recent work, using both cell culture model systems and tumour derived cell lines, suggests that the differential recruitment into polysomes of mRNA populations may be sufficient to initiate and maintain tumour formation. Consequently, a major effort is underway to use high density microarray profiles to establish molecular fingerprints for cells exposed to defined drug regimes. The aim of these pharmacogenomic approaches is to provide new information on how drugs can impact on the translational read-out within a defined cellular background.

Methods

We describe an approach that permits the analysis of de-novo mRNA-ribosome association in-vivo during short drug exposures. It combines hypertonic shock, polysome fractionation and high-throughput analysis to provide a

SUBMITTER: Genolet R 

PROVIDER: S-EPMC2533349 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature