Incorporation of enzyme concentrations into FBA and identification of optimal metabolic pathways.

ABSTRACT:

Background

In the present article, we propose a method for determining optimal metabolic pathways in terms of the level of concentration of the enzymes catalyzing various reactions in the entire metabolic network. The method, first of all, generates data on reaction fluxes in a pathway based on steady state condition. A set of constraints is formulated incorporating weighting coefficients corresponding to concentration of enzymes catalyzing reactions in the pathway. Finally, the rate of yield of the target metabolite, starting with a given substrate, is maximized in order to identify an optimal pathway through these weighting coefficients.

Results

The effectiveness of the present method is demonstrated on two synthetic systems existing in the literature, two pentose phosphat

SUBMITTER: De RK 

PROVIDER: S-EPMC2533768 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature