Hypoxia-inducible factor augments experimental colitis through an MIF-dependent inflammatory signaling cascade.

ABSTRACT:

Background & aims

Colon epithelial cells are critical for barrier function and contain a highly developed immune response. A previous study has shown hypoxia-inducible factor (HIF) as a critical regulator of barrier protection during colon epithelial injury. However, the role of HIF signaling in colon mucosal immunity is not known.

Methods

With the use of cre/loxP technology, intestinal-specific disruption of von Hippel-Lindau tumor suppressor protein (Vhl), hypoxia-inducible factor (Hif)-1alpha, and aryl hydrocarbon nuclear translocator (Arnt) was generated. Colon inflammation was induced using a dextran sulfate sodium (DSS)-induced colitis model, and the mice were analyzed by histologic analysis, Western blot analysis, and quantitative polymerase chain reaction.

Results

SUBMITTER: Shah YM 

PROVIDER: S-EPMC2533811 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature