Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae.

ABSTRACT:

Background

The Zap1 transcription factor is a central player in the response of yeast to changes in zinc status. We previously used transcriptome profiling with DNA microarrays to identify 46 potential Zap1 target genes in the yeast genome. In this new study, we used complementary methods to identify additional Zap1 target genes.

Results

With alternative growth conditions for the microarray experiments and a more sensitive motif identification algorithm, we identified 31 new potential targets of Zap1 activation. Moreover, an analysis of the response of Zap1 target genes to a range of zinc concentrations and to zinc withdrawal over time demonstrated that these genes respond differently to zinc deficiency. Some genes are induced under mild zinc deficiency and act as a first li

SUBMITTER: Wu CY 

PROVIDER: S-EPMC2535606 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature